FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310526

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310526
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12188789
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG338790.27795214110277894TruSeq Adapter, Index 10 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC211320.17337243265102054TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG43300.039.38749345
GATCGGA27000.038.772831
ATCGGAA66650.025.8042281
CGTATGC66800.025.56605543
TATCTCG66050.025.51412438
CTCGTAT66550.025.35821741
TCGTATG67300.025.30935142
TCTCGTA67000.025.28761540
TCGGAAG68250.025.2468322
ACACGTC69600.024.82126212
CGGAAGA70700.024.5946663
ATCTCGT69100.024.38845839
CGTCTGA70700.024.27599515
ACGTCTG72350.023.59799614
CACGTCT73400.023.56688513
GTATGCC74700.022.92139444
GCACACG75650.022.83621810
CACACGT77100.022.4359211
CACTAGC77900.022.1789130
GTCACTA82800.021.05588728
Produced by FastQC (version 0.11.5)