FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7039584
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCG453550.6442852304908926TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG58250.040.01504545
ATCGGAA63000.039.8295021
TCGGAAG63900.039.2268942
ACACGTC63400.039.17717712
CGTCTGA60450.038.4104215
CGGAAGA65650.038.2487033
ACGTCTG61200.037.939714
CACGTCT62150.037.4321713
CTCGTAT62350.037.10165441
ACGTCGA4250.037.05738414
GCACACG68100.036.80395510
TCGTATG63050.036.79707342
TCTCGTA57350.036.72645640
CACACGT67900.036.7135511
CGTATGC63500.036.67805543
CGTCGAA4350.036.2054915
ATCTCGT58150.036.02770239
GTCGAAC4600.034.72691316
AATCTCG61750.033.96374538
GTATGCC68700.033.9610844
Produced by FastQC (version 0.11.5)