FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005310530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142034444
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG63150.020.84380132
CGTCCGC69500.019.23128333
GATCGGA73800.018.734471
GTCCGCA81450.016.35534534
TCCGCAC86750.015.48584235
CTCGTAT87900.015.3860144
TCTCGTA88200.015.30824843
TCGTATG99200.013.74609945
ATCGGAA105750.013.3823972
CGCACAT102300.013.28616737
CGTTGGA305850.012.2142631
CCGCACA112450.012.08686736
TCGGAAG122400.012.0215373
GTCACGT116050.012.00120229
ACACGTC115500.011.97900813
ATCTCGT114000.011.96218842
AGTCACG118150.011.80711928
CGTATGC57700.011.34842945
CGTCTGA122450.011.17049316
TCACGTC129600.010.62503930
Produced by FastQC (version 0.11.5)