FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4559539
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC200470.4396716422427793TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA23900.043.0776371
ATCGGAA24600.041.9838332
ACACGTC25300.040.37355413
TCGGAAG25600.040.343843
CGTCTGA24250.039.61665316
CTCGTAT24600.039.147942
CGTATGC24850.038.75448244
TCTCGTA24850.038.7519341
ACGTCTG24900.038.67284415
CGGAAGA26600.038.5733834
TCGTATG25400.037.91530643
CACGTCT25750.037.39626714
ATCTCGT26000.036.95136640
GTATGCC27000.035.8347645
GCACACG28700.035.8250211
ACGTCGA1650.035.45284315
CACACGT29350.035.18493312
AATCTCG27700.034.9272739
CACGTCG1950.031.1523514
ACCAATC31150.030.9856736
Produced by FastQC (version 0.11.5)