FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310537

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310537
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8886431
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC1100551.2384612000025657TruSeq Adapter, Index 9 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCG408080.4592169792349707TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA117100.043.642141
TATGCCG53250.042.13081745
TCTCGTA163950.030.45238741
GTCACGA164150.030.44235429
CTCGTAT164150.030.34896942
AGTCACG165750.030.21908628
CGTATGC165650.030.18333244
TCGTATG165650.030.18282143
TCACGAT165800.030.1124330
ACACGTC168550.030.0055413
ATCTCGT166200.029.99849940
CGATCAG166450.029.994533
CGTCTGA168100.029.99302716
ACGATCA167250.029.90483732
TCGGAAG172000.029.59083
ATCGGAA172250.029.5902042
ACGTCTG170850.029.57593315
CGGAAGA172400.029.469614
GCACACG172350.029.30465111
CACGATC170500.029.28234931
Produced by FastQC (version 0.11.5)