FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11390957
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13650.031.48248345
ATCGGAA16100.027.4012281
CTCGTAT15800.027.05619243
TCTCGTA16150.027.02709442
ACACGTC16350.026.6971912
TCGTATG16650.025.53981244
ATCTCGT17300.024.84033241
CGTCTGA17750.024.59041815
TCGGAAG18200.024.1118532
CGGAAGA19750.023.0162473
ACGTCTG19050.022.79422414
CACGTCT20800.021.31005113
GCACACG21350.020.44501110
AATCTCG22750.019.28516640
CACACGT23450.018.9979111
GATCGGA5000.018.9068471
CGTTGGA28200.012.610951
ACATGTC37050.012.02384431
AGAGCAC40950.011.5384877
TCTGAAC42000.011.19591317
Produced by FastQC (version 0.11.5)