FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13360540
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGC4062883.0409549314623514TruSeq Adapter, Index 13 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAAACTCGTATGC224310.16788992061698105TruSeq Adapter, Index 13 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATG147570.1104521224441527TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC466450.043.7839445
ATCGGAA479300.043.264591
TCGGAAG483550.042.8296472
CGGAAGA486300.042.5226823
ACACGTC483750.042.47198512
CGTCTGA484750.042.3472415
CTCGTAT481700.042.2442943
ACGTCTG486900.042.14638514
TCGTATG484350.042.142644
TCTCGTA445600.042.132542
CACGTCT488200.042.0756313
GCACACG491300.041.93725210
ATCTCGT448350.041.86419741
CACACGT492550.041.83082611
AATCTCG452700.041.36393740
AGTCAAC502250.040.52144633
AGAGCAC512050.040.37847
GTCAACA504650.040.25741634
AGCACAC513900.040.1498689
GAGCACA514300.040.1361358
Produced by FastQC (version 0.11.5)