FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310543

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310543
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7891840
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA3050.014.01638842
ACGGGAT13900.013.5958249
CGTATGC3500.013.50144945
ATCGGAA3550.013.3220941
CGTTGGA21200.013.2787041
CGGGATT13700.012.15204710
AACGGGA19400.011.94473658
CTCGTAT3551.7462298E-1011.4085943
ACACGTC4152.1827873E-1110.84240712
GTCACGT4405.456968E-1210.73795128
GAAACGG24500.010.5604166
AAACGGG25250.010.5138487
AGTCACG4702.1827873E-1110.05261427
TATCTCG3909.918949E-99.80725340
GGAAACG25500.09.6169115
TCGGAAG4702.2737368E-109.5791382
CGGGATA3351.2211331E-69.40202810
CGTAGCA2453.8375787E-49.1921151
ATCTCGT4905.0567905E-109.18361141
TCGTATG4203.3125616E-89.1081244
Produced by FastQC (version 0.11.5)