FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15578655
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC225570.14479427139249185TruSeq Adapter, Index 20 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC32350.036.997245
ATCGGAA35600.033.96141
TCGTATG36200.033.2488644
CTCGTAT36000.033.18360543
TCTCGTA34650.032.98314342
TCGGAAG37350.032.4053152
TATCTCG35500.032.193440
ACACGTC37900.031.69751512
GTCACGT38250.031.46679328
ATCTCGT36750.031.0983941
AGTCACG39100.030.66765427
CGTCTGA39500.030.35661115
CGGAAGA41200.029.650173
ACGTGGC41100.029.39973831
ACGTCTG41400.029.07211314
CGTGGCC41850.028.92662632
CACGTCT42750.028.31192213
TCACGTG43450.027.80402429
GCACACG45850.026.34863310
CACACGT47300.025.49334511
Produced by FastQC (version 0.11.5)