FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310549

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310549
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84569085
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC70450.027.26878445
CTCGTAT70850.026.7655843
TCTCGTA67100.025.88115942
AGTTCCG73100.025.81870833
TATCTCG68150.025.51541140
CCGTATC70000.024.96967737
ATCGGAA77650.024.8075281
TCGTATG78850.023.96439744
TCCGTAT76200.023.05610836
ACACGTC84900.022.62763212
TCGGAAG86050.022.4155162
TTCCGTA89750.021.20439335
ATCTCGT82250.021.1960441
CGTCTGA91450.021.0807515
CGTATCT85000.020.5896838
GTTCCGT97350.019.6645334
CGGAAGA101650.019.5046313
ACGTCTG104200.018.45811314
CACGTCT113350.017.22610713
GCACACG127900.015.42474410
Produced by FastQC (version 0.11.5)