FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33290505
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31200.032.4466945
CTCGTAT33250.029.02526341
CGTATGC35800.028.46616643
ATCGGAA38150.027.743761
TCTCGTA32000.027.3478940
TATCTCG33150.026.80627638
TCGTATG37900.026.29511342
CGTCTGA41000.025.78740115
TCGGAAG41650.025.1788222
ACACGTC41550.025.1756512
ATCTCGT38000.023.44422139
CGGAAGA45850.023.2068423
ACGTCTG49100.021.48745314
CACGTCT51850.020.52159913
GCACACG59550.017.90586910
GTATGCC62900.016.6666844
CACACGT66450.016.04656611
CACTAGC69300.015.0958330
GTCACTA77000.014.08199728
ACTAGCT83850.012.61049331
Produced by FastQC (version 0.11.5)