FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10723116
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA5850.026.9321781
CGTATGC5900.018.30461345
CTCGTAT9150.017.9508644
AGTTCCG8750.017.74223334
TCTCGTA9350.016.84558743
TCCGTAT9500.016.3423737
TATCTCG10000.016.20086741
CCGTATC9650.016.08856838
TCGTATG10200.015.44077745
ATCGGAA10300.015.2940062
ATCTCGT10850.014.93167342
CGTCTGA11150.014.32745316
ACACGTC11150.014.32604913
GTTCCGT11100.014.1886935
CGTTGGA24450.014.1765691
TTCCGTA11050.014.05000136
TCGGAAG11200.013.6631023
ACGGGAT15350.013.4839269
CGTATCT12400.012.88363339
ACGTCTG12850.012.60603215
Produced by FastQC (version 0.11.5)