FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26038082
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGC348740.13393459625789642TruSeq Adapter, Index 25 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC46400.035.8314845
CTCGTAT46400.035.49207343
TCTCGTA44950.034.83516342
ATCGGAA49200.034.7170371
TATCTCG45450.034.55571440
TCGGAAG51100.033.3005262
ACACGTC50900.032.8836512
TCGTATG50300.032.56129544
CGTCTGA52250.032.16486715
ATCTCGT50300.031.30893741
CGGAAGA56150.030.7803843
ACGTCTG58500.028.84232914
CACGTCT61000.027.66026713
GCACACG64450.026.28423310
CACACGT68000.025.04447211
GTCACAC90700.018.8289528
AGAGCAC103950.016.9240997
AGCACAC109350.015.9443269
CAGTCAC108300.015.93456626
GAGCACA110900.015.7620528
Produced by FastQC (version 0.11.5)