FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659891
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCG136110.29208837717448755TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17550.040.58305745
ATCGGAA20400.035.8044471
TCGGAAG20500.035.5140652
CTCGTAT20150.035.45879741
TCTCGTA18200.034.75657740
ACACGTC20700.034.73299412
CGTATGC20500.034.63419743
TCGTATG20650.034.38261842
CGTCTGA20800.034.3499715
CGGAAGA21850.033.525153
ACGTCTG21700.033.13239714
ATCTCGT18900.032.86948839
CACGTCT21900.032.72722613
CATCTCG19800.031.60443738
GTATGCC22750.031.30692944
GATCGGA3650.030.7865451
GCACACG24000.030.23773210
CACACGT24050.029.80150411
CTTAGGC25650.027.76735532
ACTTAGG28100.025.4260431
Produced by FastQC (version 0.11.5)