FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310556

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310556
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9893989
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC10900.031.99383545
CTCGTAT13300.025.54604743
TCTCGTA13500.025.16771542
TCGTATG14500.023.42976244
ATCGGAA15650.023.1615091
AACGATC14500.022.65646237
GTCACGT15000.021.75178528
ACACGTC16150.021.73168612
TCGGAAG16700.021.6944942
AGTCACG15250.021.39509227
ATCTCGT15500.021.3394941
CGTCTGA16350.020.91534215
ACGTCTG16950.020.17497614
CGGAAGA17950.019.9326023
GATCGGA5850.019.627711
AAACGAT17450.019.59858136
TCACGTG17400.019.00941329
GAAACGA18050.018.82245335
CACGTGA18400.018.46553630
ACGATCT17950.018.17569438
Produced by FastQC (version 0.11.5)