FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8809136
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG1180841.3404719827233909TruSeq Adapter, Index 11 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC296790.33691158815121025TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG127700.043.0665145
GATCGGA33900.041.166561
ATCGGAA159700.035.165621
TCGGAAG160450.034.9304432
ACACGTC160350.034.7486812
CGGCTAC159700.034.66480332
ACGGCTA159450.034.6475431
CGTCTGA160950.034.56995415
TCTCGTA159400.034.5134140
CGTATGC159650.034.49086443
CGGAAGA162250.034.4802133
TCGTATG160250.034.37576342
ACGTCTG162150.034.34868214
CTCGTAT160300.034.31943541
GTCACGG160900.034.30819728
TCACGGC161500.034.2225429
ATCTCGT160600.034.17459539
CATCTCG160900.034.11165238
AGTCACG161400.034.08959627
CACGTCT163800.034.04387713
Produced by FastQC (version 0.11.5)