FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4609291
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG5750.037.5106245
CGTATGC6200.034.42644543
CTCGTAT6500.032.8371841
TCTCGTA5950.032.7752440
ATCGGAA6650.031.769661
TCGTATG6800.031.38881742
CGTCTGA7350.029.0396815
TCGGAAG7600.028.3831332
ATCTCGT7300.027.02469639
ACACGTC7950.026.84800712
CGGAAGA8350.025.2955463
ACGTCTG8850.024.62544314
CACGTCT9400.023.42360913
GTATGCC10500.021.18345344
CACACGT10250.021.04277211
AATCTCG9400.020.74603338
GCACACG10550.020.65691410
GTCACAC14750.014.31845528
AGAGCAC16750.013.5472997
TCTGAAC16950.013.12277817
Produced by FastQC (version 0.11.5)