FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10770798
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGC162230.1506202233112161TruSeq Adapter, Index 15 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC22250.039.3344245
CTCGTAT24500.034.8037743
TCGTATG25550.033.63764644
TCTCGTA22550.033.32275842
ATCGGAA26500.032.788041
TCGGAAG26950.032.142922
CGTCTGA27300.031.72523315
ACACGTC27850.031.1794812
ATCTCGT24600.030.5462941
CGGAAGA28700.030.4965763
ACGTCTG28750.030.12517714
CACGTCT31250.027.71516413
GCACACG32950.026.4901910
CACACGT33150.026.46597111
AATCTCG29350.025.7557540
GATCGGA5100.024.4018441
ACATGTC46350.019.31800531
AGAGCAC48750.018.2738197
TCTGAAC49950.018.01504317
AGCACAC49700.017.9245229
Produced by FastQC (version 0.11.5)