FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310566

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310566
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6525834
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG80700.12366235488061755TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11550.036.62645745
GTCACGC11600.036.4635628
CGTATGC11900.035.5459443
CTCGTAT12000.035.24945441
TCGGAAG12550.034.6182982
TATCTCG11000.034.5665938
CGCCAAT12000.034.49814232
ATCGGAA12700.034.404081
TCTCGTA11350.033.69888740
ACGCCAA12550.033.3448231
CGTCTGA13150.033.02103815
ACACGTC13200.032.7245112
TCGTATG13150.032.5092542
ATCTCGT12050.031.92798639
AGTCACG13750.031.41649227
ACGTCTG14600.029.74063914
CGGAAGA14700.029.7084433
GCACACG15300.028.52656610
CACGTCT16400.026.3392413
CACACGT16600.026.15702811
Produced by FastQC (version 0.11.5)