FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9744310
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGC134900.13843976638674263TruSeq Adapter, Index 15 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC20300.037.68390345
GATCGGA11950.034.8449251
ATCGGAA31600.026.3542331
CTCGTAT29300.026.34117543
TCTCGTA29500.026.1628642
TCGTATG30200.025.48127644
ATCTCGT31100.025.10627241
ACACGTC31550.025.10078612
TCGGAAG33250.024.9064392
CGTCTGA32250.024.55634115
CGGAAGA34000.023.9599083
ACGTCTG33700.023.83356514
CACGTCT34750.022.98357613
AATCTCG34200.022.6329440
CACACGT37700.021.18501711
GCACACG37650.021.15296210
ACATGTC49100.016.4507631
AGAGCAC50100.016.2556297
AGCACAC53150.015.1534899
CCAGTCA53850.015.08323625
Produced by FastQC (version 0.11.5)