FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310570

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310570
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4946494
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAT2054.8165875E-612.07727051
CGTTGGA12200.011.6227981
AGTCCGG1400.00525533711.251430535
AACGGGA11600.09.3093638
CCATCGA1950.0057098139.2339041
GGAAACG15150.09.208445
AAACGGG13950.09.0313077
GAAACGG14850.08.9384526
CGGGAAT5058.931238E-108.90995210
CACCGCG4051.8436549E-78.88966130
CGCGCCC3807.01466E-78.88252833
CCCCGAA2555.755281E-48.8265261
ACGGGAT8200.08.7795629
GATTTCG6003.45608E-118.6254865
ACCGCGC3951.2244654E-68.545217531
CCCGTAA2450.0039351038.2681121
CCGCACT2450.00394660378.2654372
ACAGGCG19600.08.03470518
GCGTGAG13900.07.9310622
CGTGAGC14300.07.86670223
Produced by FastQC (version 0.11.5)