FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310571

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310571
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5936626
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA13350.015.3552431
CGGGAAT5850.011.92121110
AACGGGA14100.011.80666358
AAACGGG16850.011.3483727
GAAACGG17050.011.3473896
TACACGG2452.9001822E-611.02109730
ACGGGAT10350.010.8678659
GGAAACG18250.010.2317925
CGGGATT8800.09.71438910
ATAGGCG3053.920903E-58.85114918
CGGGATA3053.921291E-58.85107310
CGTATGC2400.00327302848.43859744
CTACACG3003.1652595E-48.25051529
GTTGGAA30400.08.1406542
ACGGGAA7303.6379788E-128.01244459
TCGACAG2550.00566556357.940000512
CCGTGCC6905.2641553E-97.174301632
CGTGAGC14550.07.11246923
GCGTGAG14550.07.11234922
ACAGGCG19650.07.09818618
Produced by FastQC (version 0.11.5)