FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310576

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310576
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18188228
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCG371050.20400557987287163TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG48400.040.4401545
CTCGTAT51300.038.24176841
CGCCAAT51250.038.23980332
TCTCGTA49950.037.92953540
ATCGGAA52300.037.871551
GTCACGC52450.037.53682328
CGTATGC52300.037.42453843
TATCTCG51300.037.0619638
TCGTATG53000.036.9302542
ACACGTC54050.036.62805612
ACGCCAA53750.036.50367431
ATCTCGT53150.035.8142739
TCGGAAG55650.035.7169532
CGTCTGA56000.035.19298615
AGTCACG56800.034.85996227
CGGAAGA58150.034.365413
ACGTCTG59450.033.3388814
CACGTCT60300.032.94354213
GCACACG63700.031.14985810
CACACGT66350.030.14308711
Produced by FastQC (version 0.11.5)