FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5986228
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6600.035.45804245
CGTATGC6850.034.1602443
TCGTATG6950.033.66844642
CTCGTAT7750.030.48280141
CGTCTGA8150.029.26155715
TCTCGTA7900.028.76433440
ACACGTC8300.028.7327312
ATCGGAA8300.028.2191221
TCGGAAG8850.026.4523452
CGGAAGA8950.026.4087373
ACGTCTG9400.025.60973514
ATCTCGT9050.024.3633839
CATCTCG9600.022.9675638
CACGTCT10400.022.9309313
GTATGCC11100.022.09677344
GCACACG11150.021.38813410
CACACGT12250.019.83487911
CTTAGGC14200.016.9534932
GCGACTA800.001712861716.87370312
CGTTGGA13200.013.9903391
Produced by FastQC (version 0.11.5)