FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8417146
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC571080.6784722517584939TruSeq Adapter, Index 20 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATG364780.4333772991462902TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC70500.041.2721845
CGTCACG5400.036.64385627
TCTCGTA105450.027.20923442
CTCGTAT105750.027.13204643
TCGTATG107850.026.9998844
TATCTCG105700.026.74068540
ATCTCGT106850.026.45288341
GTCACGT110750.026.2522828
CGTGGCC111600.026.07263432
ACGTGGC112050.025.90756631
AGTCACG106200.025.53495227
TCACGTG113000.025.51066829
GATCGGA65900.025.030981
GGCCTTA115450.024.91116335
CACGTGG116050.024.87895430
GCCTTAT119850.024.35294336
CGTCTGA121350.024.218415
CCTTATC118200.024.08413337
ACACGTC123500.023.97885712
ACGTCTG123700.023.86737614
Produced by FastQC (version 0.11.5)