FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8579643
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG108330.12626399490048712TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15500.039.33614345
CTCGTAT16500.036.54754341
CGATGTA16750.035.728833
TCTCGTA15150.035.34855740
ACCGATG17250.035.3453131
CGTATGC17350.035.146343
CCGATGT17250.034.95403732
ATCGGAA17550.034.6246261
CACCGAT17650.034.41882330
TCGGAAG17750.033.975752
TATCTCG15700.033.5369538
GTCACCG18400.033.38291528
ACACGTC18400.033.25850712
CGTCTGA18800.033.0311115
TCGTATG18450.032.68498242
TCACCGA19150.031.95781129
CGGAAGA19700.030.7256413
ATCTCGT17600.030.6835739
ACGTCTG20700.029.89057414
CACGTCT21800.028.48554213
Produced by FastQC (version 0.11.5)