FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310581

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310581
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8892147
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGC214220.24090919774493158TruSeq Adapter, Index 20 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC26450.039.4734545
TCTCGTA24950.037.42724242
CTCGTAT28100.036.91517343
ATCGGAA29350.036.9130361
ACACGTC28850.036.88791312
TCGGAAG30050.036.013842
TATCTCG25900.035.9653340
CGTCTGA29750.035.6963515
AGTCACG29250.035.69117727
TCGTATG29300.035.4034944
ATCTCGT26450.035.132641
GTCACGT29600.034.81308728
CGGAAGA31800.034.1734473
ACGTCTG31800.033.60742614
CACGTCT32100.033.08306513
CGTGGCC31800.032.5461432
ACGTGGC32300.032.18164431
GCACACG33850.031.57211910
TCACGTG33400.031.18913529
CACACGT35450.030.2740811
Produced by FastQC (version 0.11.5)