FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13234706
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCG296560.22407751256431385TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37250.041.44374545
CGGCTAC38600.039.63744432
ACGGCTA39800.038.4988831
CGTATGC40300.038.2428243
CTCGTAT40250.038.1226341
ATCGGAA41600.037.5125771
TCTCGTA37100.037.41700740
GTCACGG42050.036.91837328
TCGTATG41850.036.8801842
TCGGAAG42550.036.606622
ACACGTC42400.036.4012312
CGTCTGA43450.035.46978815
AGTCACG43650.035.3072727
ATCTCGT38850.035.2682639
CGGAAGA44450.034.9351923
TCACGGC44200.034.86805729
ACGTCTG45250.034.05883814
CATCTCG40700.033.7204438
CACGGCT46250.033.27390730
CACGTCT46500.033.2400413
Produced by FastQC (version 0.11.5)