FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7045384
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCG107930.15319250164362938TruSeq Adapter, Index 2 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC98410.13968010828082614TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG12750.037.23520345
GATCGGA12950.035.812771
CACCGAT23200.020.46244430
TATCTCG23250.020.32253338
ACCGATG23150.020.31226231
TCTCGTA23650.020.2640840
CTCGTAT23600.020.11647841
CGATGTA23600.019.92509533
CCGATGT24000.019.68647832
CGTATGC24300.019.62930343
ATCGGAA24250.019.4961131
ATCTCGT24350.019.4044739
ACACGTC24500.019.3761312
GTCACCG24750.019.27228
TCGTATG24800.019.14255742
TCACCGA24800.019.05156329
CGTCTGA25250.018.80060215
TCGGAAG25150.018.79072
ACGTCTG26600.017.93101514
CGGAAGA26550.017.7998523
Produced by FastQC (version 0.11.5)