FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15795294
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG293570.18585915526485294TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37100.038.93254545
GATCGGA12600.032.7223471
CGTATGC47050.030.84265343
CTCGTAT47300.030.72720341
TCGTATG47750.030.43762842
ATCGGAA49000.030.254761
TCTCGTA46600.029.98490740
ACACGTC49300.029.8445212
TCGGAAG49800.029.7857482
CGTCTGA50650.029.13863615
ATCTCGT48400.028.82209239
ACGTCTG52000.028.55448714
CGGAAGA52450.028.3172113
CACGTCT54350.027.23705113
GCACACG58400.025.57932310
GTATGCC58200.024.8951444
CACACGT59950.024.84291811
AATCTCG57000.024.39454738
AGTCACC80150.018.7244827
AGAGCAC81650.018.378227
Produced by FastQC (version 0.11.5)