FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310592

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310592
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28909365
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC940010.32515760896166346TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA115500.039.918941
CTCGTAT126650.035.40715842
TCTCGTA126850.035.40454541
CGTATGC128100.035.14792644
TATCTCG127400.035.0919639
ATCTCGT130550.034.40035240
TCGTATG131000.034.30113643
ATCGGAA135550.034.1439672
CGTCTGA135100.033.8746516
ACACGTC135350.033.84532513
TCGGAAG138900.033.320483
ACGTCTG141650.032.32414615
CGGAAGA144950.031.97634
CACGTCT146600.031.294114
TATGCCG24700.030.79083645
GCACACG151950.030.2811111
GTATGCC149350.030.14696545
CACACGT157000.029.37875612
CACTAGC155100.029.27453831
GCTTATC161800.027.9092936
Produced by FastQC (version 0.11.5)