FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14116820
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16800.036.16129745
GATCGGA9850.027.1824111
CGTATGC24300.025.27863343
ATCGGAA25800.024.1566831
TCTCGTA25300.024.01258740
CTCGTAT25250.023.9709441
TCGTATG25750.023.76779442
TCGGAAG27050.023.1233982
CGTCTGA27300.022.99406215
ACACGTC27500.022.9086512
ATCTCGT27000.022.33393139
ACGTCTG29600.021.43539814
CGGAAGA30200.020.9350283
CACGTCT31500.020.49960313
AATCTCG32250.018.83764638
GCACACG34400.018.64062910
GTATGCC34100.018.54135544
CACACGT35600.018.01229311
AGTCACC50600.013.16208227
GTCACCT51200.012.78811228
Produced by FastQC (version 0.11.5)