FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12911630
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTATGC208040.16112605457250556TruSeq Adapter, Index 27 (97% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC28300.037.8395645
ATCGGAA31350.034.9948731
TCTCGTA28300.034.81875642
CTCGTAT30600.034.48077443
TATCTCG28800.034.3707640
TCGGAAG32550.033.811032
ACACGTC32400.033.673412
CGTCTGA32600.033.25993715
TCGTATG31800.033.10886844
CGGAAGA33700.032.8575943
ATCTCGT30550.032.47490741
ACGTCTG34900.031.32584614
CACGTCT35250.031.07850513
GCACACG37200.029.38971310
CACACGT39600.027.8353211
AGAGCAC56000.019.8048277
TCTGAAC59500.018.60116417
GAGCACA60750.018.589588
AGCACAC60350.018.5259789
GAAGAGC62750.018.2493825
Produced by FastQC (version 0.11.5)