FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7460524
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCG203760.2731175450946877TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28100.040.59864445
GATCGGA9650.036.6470871
CTCGTAT35500.031.9443941
ATCGGAA36150.031.715871
CGTATGC36050.031.58291443
TCGGAAG36450.031.2951242
TCTCGTA33650.030.82516140
TCGTATG37050.030.73047442
ACACGTC37300.030.34068512
TATCTCG34050.030.33007238
CGTCTGA37500.030.23866315
ATCTCGT34350.030.13068239
CGGAAGA37900.030.0382483
ACGTCTG38550.029.47340214
GCACACG39700.028.84652310
CACGTCT39650.028.76941313
CACACGT41150.027.5566811
GTATGCC42200.027.08701544
CACTAGC43600.026.1115830
GTCACTA44050.025.74268228
Produced by FastQC (version 0.11.5)