FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40375756
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC39600.030.52226345
CTCGTAT40600.029.32823443
TCTCGTA38950.028.66718342
TCGTATG42750.027.85283344
AGTTCCG42900.027.68267633
ATCGGAA44500.027.211381
CCGTATC42000.026.48015437
TCCGTAT42400.026.40597336
TATCTCG42750.025.75277340
TTCCGTA47700.024.94491835
TCGGAAG48700.024.9196112
ACACGTC49950.024.33333412
CGTATCT46350.024.14038538
GTTCCGT49700.023.9863134
CGTCTGA51600.023.5111415
ATCTCGT48350.023.0488541
CGGAAGA57200.021.4867083
ACGTCTG57000.021.36259714
CACGTCT64550.019.14283613
GCACACG68400.018.09819810
Produced by FastQC (version 0.11.5)