FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15367819
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC522130.33975543309040795TruSeq Adapter, Index 5 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCG263510.17146870352910845TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA62750.042.095841
TATGCCG33600.037.0984145
ATCGGAA92400.028.5871622
CTCGTAT93000.027.94442442
CGTATGC93450.027.90553344
TCGGAAG95000.027.8758283
TCGTATG93400.027.87292943
TCTCGTA93650.027.7504741
ACACGTC96500.027.32576813
CGTCTGA96150.027.30823916
ATCTCGT96050.027.03364240
CGGAAGA98450.026.830414
ACGTCTG99500.026.38881515
CACGTCT102200.025.8017314
GTATGCC101600.025.7555545
GCACACG103900.025.35790811
CACACGT107000.024.68632312
GATCTCG114550.022.74631339
GTCACAC118750.022.16839429
AGAGCAC128000.020.7241128
Produced by FastQC (version 0.11.5)