FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9717281
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGC115890.11926175645224214TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG16700.037.31851632
CGTACGT16750.037.207533
CGTATGC17650.035.8217945
ACCGTAC17850.035.16669531
CTCGTAT17900.034.56693343
TCTCGTA18550.033.35500742
GTACGTA19200.032.6940334
TCGTATG19350.032.20953444
CGTAATC19500.031.61496237
ATCGGAA20500.030.7501261
GTCACCG20650.030.72550628
ATCTCGT20150.030.707141
TCGGAAG20600.030.6986032
CACCGTA20950.029.85563330
ACGTAAT21150.029.78683736
ACACGTC21150.029.78499612
CGTCTGA21650.029.09712215
TACGTAA22450.027.96176535
ACGTCTG22700.027.94944414
CGGAAGA23300.027.2378433
Produced by FastQC (version 0.11.5)