FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8012889
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7450.030.80749345
CTCGTAT8750.025.45889743
TCGTATG9500.023.44898444
TCTCGTA8350.022.9057742
TCGGAAG10050.022.8349722
GATCGGA3650.022.8334471
ATCGGAA10500.021.6667941
ACACGTC10500.021.213112
CGTCTGA11000.020.862615
ATCTCGT10450.018.51744541
CGGAAGA13200.017.38553
ACGTCTG13100.017.34646814
GCACACG13950.016.45069310
CACGTCT13900.016.34800713
AATCTCG13450.014.88903740
CACACGT16000.014.764811
CGTTGGA15700.012.9123551
AAACGGG20250.010.3327567
AACGGGA16300.010.2141528
GAAACGG20600.09.9387046
Produced by FastQC (version 0.11.5)