FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7030223
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG578310.8226054849184727TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG71350.043.0792245
ATCGGAA77300.040.509461
TCGGAAG78000.040.0642472
TCGTATG76950.039.82718742
CTCGTAT76950.039.79681441
CGGAAGA79100.039.5924223
CGTATGC77650.039.55508843
ACACGTC78750.039.48354712
TCTCGTA68500.039.48323440
CGTCTGA79200.039.11660815
ATCTCGT69050.039.03700339
ACGTCTG79800.038.9070814
CACGTCT81350.038.24927513
CACACGT83000.037.5973311
GCACACG82750.037.57497410
AATCTCG72300.037.2822338
GTATGCC82600.037.1846544
GATCGGA6350.035.1222761
AGTCACC89900.034.23713327
AGCACAC93050.033.705839
Produced by FastQC (version 0.11.5)