FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7119192
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCG176560.24800567255385159TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23650.041.10087245
ATCGGAA25500.039.3130871
CGTATGC24950.039.04952643
TCGTATG25000.038.7911542
TCGGAAG25750.038.7066232
CTCGTAT25100.038.4565141
TCTCGTA21500.037.98896840
CGTCTGA26150.037.42704415
ACACGTC26400.037.24333212
CGGAAGA27100.036.8614543
TCACGAT22800.035.8216734
ATCTCGT22900.035.4687439
GCACACG28350.035.07818610
CATCACG27800.034.7200632
ACGTCTG28400.034.46187214
CACGTCT29050.033.8459213
ACGATCT24850.032.50462336
CACACGT30600.032.3520811
CACGATC25250.032.25679835
GTATGCC30800.031.70570844
Produced by FastQC (version 0.11.5)