FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8549140
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG7850.035.5398745
ATCGGAA9750.029.0821341
CTCGTAT9700.028.997541
CGTATGC9850.028.55624643
TCTCGTA8800.028.3832840
ACACGTC10350.027.38948412
TCGTATG10500.026.78847742
TCGGAAG10750.026.582742
CGTCTGA10850.025.7134815
TCACGAT10250.024.14502134
ATCTCGT10400.023.80025339
ACGTCTG12550.022.40965714
CATCACG12550.022.229632
CGGAAGA13050.022.0696953
CACGTCT13700.020.52855313
GCACACG15000.019.34871510
CACGATC13050.018.79207635
ACGATCT13050.018.6196736
GTATGCC15800.018.22706444
CACACGT15900.017.97050511
Produced by FastQC (version 0.11.5)