FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85850758
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA39900.020.105121
CGTATGC43050.016.93452845
CGTTTCG48200.016.61833433
CTCGTAT58450.013.78263944
TCTCGTA59350.013.57365943
CGTTGGA199800.013.2742671
TCGGAAT63400.012.81282837
GTTTCGG64450.012.70861434
ATCGGAA65600.012.6894042
TCGTATG65750.012.42258945
TTCGGAA68500.011.99018336
ACGGGAT135950.011.7989479
AACGGGA180700.011.653368
AAACGGG223400.011.57099157
TCGGAAG73350.011.4099543
ACACGTC71900.011.38945513
GAAACGG232200.011.2003976
GTCACGT75550.010.9589429
ATCTCGT74650.010.88208942
AGTCACG77700.010.77155528
Produced by FastQC (version 0.11.5)