FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19489689
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCG1314400.6744078881915458TruSeq Adapter, Index 7 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG152500.042.8399445
ATCGGAA170800.039.1502231
CTCGTAT168100.038.70361741
TCTCGTA160650.038.63657840
TCGGAAG172900.038.584182
CGTATGC169600.038.45425843
ACACGTC172300.038.39700312
TCGTATG170300.038.29649442
CGTCTGA173400.038.10162715
CGGAAGA176650.037.777843
ACGTCTG175150.037.6181914
ATCTCGT165500.037.3955839
CATCTCG166750.037.15572738
CACGTCT178350.037.00620313
GCACACG182500.036.25255610
CACACGT184150.035.9631811
GTATGCC183050.035.7147844
GATCGGA19650.034.3061031
GATCATC190400.032.6701735
AGAGCAC208150.032.077517
Produced by FastQC (version 0.11.5)