FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310610

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310610
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13312334
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC11350.029.93131645
CTCGTAT11750.028.52911243
TCGTATG12850.026.4373944
TCTCGTA11150.026.43327342
GTCACGA13150.026.1758428
ATCGGAA13550.025.6212181
TCGGAAG14250.025.109872
ACACGTC14050.024.49900412
TATCTCG12400.023.76970340
ACGAGTG14700.023.72198131
CACGAGT14450.023.66513830
AGTCACG14950.023.17471927
CGTCTGA15350.022.4241715
ACGTCTG17150.020.20179414
CGGAAGA17850.019.6674753
ATCTCGT15050.019.43389141
CACGTCT18650.019.18013213
CACACGT21900.016.43649511
GCACACG22450.015.83416910
CGAGTGG23150.014.96697532
Produced by FastQC (version 0.11.5)