FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21440645
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC19800.031.01891145
GATCGGA12200.025.9199221
CTCGTAT25500.023.29217543
TCGTATG27650.021.80639844
TCTCGTA24050.020.767542
ATCGGAA29400.020.6666661
CGTCTGA30700.019.93235815
ACACGTC30550.019.6620212
TCGGAAG31050.019.569842
ATCTCGT27400.018.22840941
CGGAAGA34950.017.3797153
ACGTCTG35100.017.11324114
CACGTCT37450.016.0393813
GCACACG41950.014.80148110
CACACGT43750.014.089663511
ACTCGTA5850.013.84542
AATCTCG39500.012.75846140
CGTTGGA50400.011.8314741
ACGGGAT33600.010.9807669
AAACGGG55700.010.50132757
Produced by FastQC (version 0.11.5)