FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9021774
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20000.012.092441
GAAACGG22950.010.7826196
AAACGGG22050.010.7126027
AACGGGA18550.010.6721778
ACGGGAT12900.010.2890819
GGAAACG23600.010.009025
CGGGATT12450.09.93819710
AACGGGT4254.03179E-88.9985868
CGTATGC3104.515303E-47.98266145
CGGGAAT9350.07.93992910
ACAGGCG33800.07.85379518
CCCCGAA4152.0472491E-57.6250051
AGGCGCC10700.07.56890420
GCGTGAG25100.07.52890622
ACGCCCG6602.1464075E-97.501107734
CGTGAGC26550.07.45666223
CACCGCG10200.07.27826830
ACTGCGC9900.07.273801331
CCCAAAG98200.07.18130351
CCTCGAT3150.00443228337.17545751
Produced by FastQC (version 0.11.5)