FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6415505
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC3439945.3619161702781TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA389750.044.8572581
ATCGGAA390750.044.736012
TCGGAAG391050.044.6132663
ACACGTC391300.044.193313
CGGAAGA394200.044.184514
CGTCTGA392700.043.91577516
CACGTCT395000.043.7907314
ACGTCTG394650.043.78429815
GCACACG396250.043.76614811
CACACGT396700.043.6824712
TCTCGTA377600.043.6642641
CTCGTAT376300.043.56398442
TCACGAT390400.043.5347935
ATCTCGT380350.043.47870340
CGTATGC373700.043.45733344
TCGTATG376200.043.33633843
CATCACG395350.043.2856433
ACGATCT391300.043.22837
GTATGCC375950.043.20322845
CACGATC392850.043.17165436
Produced by FastQC (version 0.11.5)