FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25390366
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGC518660.20427432987771818TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC69450.038.35534345
ATCGGAA78600.034.4896551
TCTCGTA74150.034.1643942
CTCGTAT77550.034.11708543
GTCACGA78250.034.01676628
TATCTCG74800.033.72176440
TCGGAAG80450.033.5485042
ACACGTC80700.033.0068512
TCGTATG80150.032.9822944
CACGAGT81750.032.61543730
CGTCTGA82650.032.17461415
AGTCACG83350.032.15131827
ATCTCGT79000.032.06695641
ACGAGTG83300.032.0625731
CGGAAGA85550.031.7770773
ACGTCTG85350.031.34043914
CACGTCT89350.029.93733813
GCACACG92750.029.0339510
GATCGGA14850.028.9836221
CACACGT97700.027.6089911
Produced by FastQC (version 0.11.5)