FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8960952
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCG96700610.791331099642091TruSeq Adapter, Index 12 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC7759938.659716065882286TruSeq Adapter, Index 12 (100% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGCCG400800.447273905718946TruSeq Adapter, Index 12 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAGCTCGTATGCCG308430.34419334017189246TruSeq Adapter, Index 12 (98% over 51bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGCCG250120.27912212898808075TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAGCTCGTATGCC194470.21701935240809236TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAACCTCGTATGCC148910.16617654017117825TruSeq Adapter, Index 12 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAAACTCGTATGCC127080.1418152892683724TruSeq Adapter, Index 12 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA870150.045.108631
TATGCCG1176400.043.4089545
ACTCGTA66600.031.98971440
AAACTCG68100.031.41653838
AACTCGT69050.030.72434239
CCGTCTT15900.027.59206245
GCTCGTA61000.026.96013840
AGCTCGT62050.026.5401839
AAGCTCG61850.026.44353938
GCCGTCT16450.026.25923244
GTAAACT82250.025.95704336
ATCGGAA2018550.025.6006011
TGTAAAC84500.025.55945235
TCGGAAG2025050.025.4754732
CGGAAGA2027650.025.3950943
TAAACTC84300.025.29913537
ACACGTC2034500.025.20544412
ACGTCTG2033100.025.18407414
CGTCTGA2031400.025.1752515
CACGTCT2036400.025.17419213
Produced by FastQC (version 0.11.5)