FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005310618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005310618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9746178
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC13750.033.7075345
GATCGGA7750.030.8203681
CCGTACG16050.028.87776232
CGTACGT16500.028.09018533
ACCGTAC17500.026.74216831
CTCGTAT18300.025.32656543
GTCACCG19200.025.07773828
GTACGTA18600.025.03967734
ATCGGAA19650.024.1965561
TCGTATG19350.023.9523844
TCGGAAG20150.023.6805952
ACACGTC20450.023.21182312
CACCGTA20650.023.20858830
TCTCGTA16350.022.98176842
TACGTAA20850.022.44546335
CGTAATC16700.022.3658437
CGTCTGA21600.022.18465215
ACGTCTG22700.021.10929914
CGGAAGA23150.020.8062883
ATCTCGT17800.020.73044241
Produced by FastQC (version 0.11.5)